Journal: Journal of Biomedical Materials Research. Part a

Article Title: Photocurable antimicrobial silk‐based hydrogels for corneal repair

doi: 10.1002/jbm.a.37381

Figure Lengend Snippet: Experimental outline investigating the properties of gentamicin‐loaded SilkMA hydrogels. (1) Photocrosslinking of 15%, 20%, and 25% gentamicin‐loaded SilkMA hydrogels. The transparency in the visible range, swelling, in vitro degradation, porosity/pore size, compressive Young's modulus, and rheological properties of the SilkMA hydrogels were studied. (2) In vitro cytocompatibility of gentamicin‐loaded SilkMA hydrogels. Firstly, the cytocompatibility of SF and SilkMA prepolymer solutions and SilkMA hydrogels was assessed. Then, the ability of human dermal fibroblast (HDF) cells to attach and proliferate on SilkMA hydrogels was studied and compared with GelMA hydrogels. (3) In vitro gentamicin drug release from SilkMA hydrogels. The cumulative drug release of gentamicin from SilkMA hydrogels and gentamicin concentration in the media were calculated. (4) Antimicrobial testing of gentamicin‐SilkMA hydrogels. The antimicrobial activity of SilkMA hydrogels against two of the most common causes of ocular infections, Staphylococcus aureus and Pseudomonas aeruginosa was assessed in vitro

Article Snippet: Human Dermal Fibroblasts (HDFs—ATCC Cat. PCS‐201‐012; Passage number 9) were cultured in Dulbecco's modified eagle medium (DMEM—Sigma‐Aldrich, UK) and supplemented with 10% fetal bovine serum (Sigma‐Aldrich, UK), 1% penicillin/streptomycin (Sigma‐Aldrich, UK) and 2 mM L‐glutamine (Sigma‐Aldrich, UK).

Techniques: In Vitro, Pore Size, Concentration Assay, Activity Assay